Analysis of variance performed amongst different herbs showed statistically significant difference p curry leaves, cardamom, tulsi stem and tulsi leaves. Pdf antimicrobial activity of ocimum sanctum leaves extracts and oil. Antimicrobial activity of herbal medicines tulsi extract. The roots, leaves and seeds of tulsi possess several medicinal properties. A holy plant with high medicinal and therapeutic value.
The aims of this project were to examine the antimicrobial properties of essential oils distilled from australiangrown ocimum tenuiflorum tulsi, to quantify the. Varying concentration of each extracts 200mgml, 150 mgml, 100mgml. Azadirachta indica neem is a multipurpose tree with multiple health benefits. This study was focused on to compare the antimicrobial activity of methanolic leaf extracts of tulsi and neem.
In vitro antibacterial activity of some plant essential oils. In india two forms of tulsi are more common dark or shyama krishna tulsi and light or rama tulsi. Specific antibacterial activity of ocimum sanctum against. Antibacterial activity of garlic, tulsi, bitter guard and cinnamon extracts against wound pathogens godfrey omare mauti, eliakim mbaka mauti, geoffrey arasa ouno, benard maronga mabeya in the present study antibacterial properties of fruits harvested from natural medicinal plants garlic, tulsi, bitter. The tulsi with dark green or purple leaves and purple stem is called shyamatulsi dark tulsi or krishnatulsi dark tulsi. Pdf study of invitro antioxidant and antibacterial. Antimicrobial activity of neem, tulsi, henna and amla against pathogenic bacteria seema rawat department of botany and microbiology, h. Antimicrobial activity of aloe barbadensis miller leaf gel components, j. The antibacterial activity associated with the contribution of the menthol iscan et al. Khalid mahmood1, uzma yaqoob1 and rukhsana bajwa2 1govt. In vitro evaluation of antibacterial activity of various crude leaf extracts of indian sacred plant, ocimum sanctum l. Antibacterial activity of garlic, tulsi, bitter guard and cinnamon extracts against wound pathogens godfrey omare mauti, eliakim mbaka mauti, geoffrey arasa ouno, benard maronga mabeya in the present study antibacterial properties of fruits harvested from natural medicinal plants garlic, tulsi, bitter guard. Study of antibacterial activity of ocimum sanctum extract. Ocimum sanctum tulsi is a medicinal herb abundantly found and cultured in india, malaysia, australia, west africa, and some of the arab countries.
O 2008 antifertility potential of neem flower extract on adult female sprague dawley rats. Materials and methods collection of samples the medicinal plants used for the experiment were leaves of ocimum sanctum tulsi, flower of eugenia caryophyllata clove. Antibacterial activity of emblica officinalis is determined by the cup diffusion method and disc diffusion method as mentioned by adwan and mhanna 2008 1 and ahmad et al 2010 2 alone and in combination with antibiotics i. Effect of ocimum sanctum on noise induced changes in neutrophil function. Antibacterial activity of garlic, tulsi, bitter guard and cinnamon extracts against wound pathogens godfrey omare mauti, eliakim mbaka mauti, geoffrey arasa ouno, benard maronga mabeya in the present study antibacterial properties of fruits harvested. This study was carried out to observe the antibacterial activity of aqueous extract, chloroform extract, alcohol extract and oil obtained from leaves of ocimum sanctum against the selected bacteria i. Ai has several active constituents like nimbidin, nimbin, nimbolide. Klebsiella the best antimicrobial activity was observed with eucalyptus leaf ethanol extracts. Ocimum sanctum also possesses antifungal activity against asperigillus niger and aqueous extract of it was found to be effective in patients suffering from viral encephalitis. This study concludes that the neem, tulsi and cow urine has good antioxidant and antibacterial activity individually as well as synergistically and the effect is concentrationdependent. Nov 06, 2015 the dried extracts were then labeled and kept in airtight bottles in the refrigerator at 4 c prior to determination of antibacterial activity.
Pdf antibacterial activity of garlic, tulsi, bitter guard. Review article chemical constituents and pharmacological. Phytochemical analysis, antibacterial and antioxidant activity determination of ocimum sanctum. Evaluation of some chemical composition, minerals fatty. The nutritional analysis of ocimum sanctum shown high level of ascorbic acid and total carbohydrate i. The medicinal plants are widely used by the traditional medical practitioners for curing various diseases in their day to day.
It has a variety of biological pharmacological activities such as antibacterial, antiviral, antifungal. Its root bark was found to have antibacterial activity against both gram positive and gram negative bacteria. Antibacterial activity of essential oil of ocimum sanctum l. Antimicrobial activity of tulsi ocimum tenuiflorum essential oil and their major constituents against three species of bacteria article pdf available in frontiers in microbiology 7660 may. May 17, 2016 the aims of this project were to examine the antimicrobial properties of essential oils distilled from australiangrown ocimum teniflorum tulsi, to quantify the volatile components present in flower spikes, leaves and the essential oil, and to investigate the compounds responsible for any activity. Antimicrobial activity refers to the ability of a substance to reduce or inhibit the growth of microorganisms. Some of them act as bactericides which kills bacteria and some act as bacteriostatic interfere with the multiplication, growth or activity of bacteria 3. We were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. Higher antibacterial activity was shown against gram negative bacteria compared to gram positive bacteria tested. Mishra and mishra 15 have observed antibacterial activity of. Antibacterial and antioxidant activities of mentha piperita l. Tulsi for exploitation of this herb in the therapy of bovine mastitis. Most effective an antibacterial activity were observed in tulsi, neem and wheat. Of these, the agar diffusion method was commonly used in earlier studies.
The present investigation aims at developing an ecofriendly natural antibacterial finish from plant. We have chosen tulsi ocimum sanctum and neem azadirachta. Phytochemical extraction and antimicrobial properties of. An antibacterial activity was observed in all herbs used. Joshi et al 14 studied antibacterial property of different medicinal plants viz. Tulsi the wonder herb pharmacological activities of. Antibacterial finish for cotton fabric from herbal products. Nov 20, 2017 from both the methods employed for analyzing the antibacterial activity of agnps, it can be inferred that agnps obtained using tulsi extract and quercetin has almost same antibacterial potential. It was found that the distilled concentrations of essential oil inhibited the growth of microorganisms and the results were comparable with those of antibiotic gentamycin. Antimicrobial activities of alcoholic extracts of neem leaves were used. The ethanolic extract of henna exhibited maximum antimicrobial potential against e.
Pdf comparative study on antimicrobial activity of tulsi ocimum. Antibacterial activity of emblica officinalis is determined by the cup diffusion method and disc diffusion method as mentioned by adwan and mhanna 2008 1 and ahmad et. Therapeutic uses of ocimum sanctum linn tulsi with a note on eugenol and its pharmacological actions. The bacteriostatic and bactericidal activity of silver nanoparticles against escherichia coli, enterococcus faecalis, and salmonella typhi was determined using bacterial growth inhibition method. Evaluation of antimicrobial activity of different solvent. Pdf antimicrobial activity of tulsi ocimum tenuiflorum. Tulsi leaves have been traditionally used for treatment of many infections. Antimicrobial activity of ocimum sanctum extract was carried out using well. All extracts of medicinal plants showed good antibacterial property table 3. Many in vitro, animal and human studies attest to tulsi having multiple therapeutic actions including adaptogenic, antimicrobial, antiinflammatory, cardioprotective, and immunomodulatory effects, yet to date there are no systematic. The holy tulsi, also known as the queen of herbs is the most sacred of all the herbs found in india.
Antimicrobial activity of tulsi ocimum tenuiflorum. Introduction nature has been a source of medicinal agents since ancient times. The herbs and spices used in traditional indian food preparations table 1 are selected for the study with the aim to spread the awareness of the utility of herbs and spices among the masses with the following objectives. Antibacterial activity of azadirachta indica neem leaf. The clinical efficacy and safety of tulsi in humans.
Tulsi, also known as holy basil, is indigenous to the indian continent and highly revered for its medicinal uses within the ayurvedic and siddha medical systems. Review article tw cleanse community welcome to your tulsi. Our results show bacteriostatic activity of tulsi oil at levels of 2. In the present study antibacterial properties of fruits harvested from natural medicinal plants garlic, tulsi, bitter guard and cinnamon, were explored against staphylococcus aureus, escherichia coli, and pseudomonas aeruginosa, which had been. In vitro evaluation of antibacterial activity of various. No antifungal activity was detected for henna aqueous solutions5. Antibacterial activity of leaf extracts of ocimum sanctum. In traditional system of medicine, different parts leaves, stem, flower,root, seed and even whole plant of ocimum. Sanctum against known bacterial with contact time 30 minutes under invitro conditions tulsi o. Synthesis of silver nanoparticles from the aqueous extract of. Emblica officinalis amla leaf extract potentiates p. In vitro antibacterial activity and flavonoid contents of.
Escherichia coliatcc 25923, staphylococcus aureusatcc. The antibacterial activity of extracts of nine plant. Evaluation of some chemical composition, minerals fatty acid. The tulsi with dark green or purple leaves and purple stem is called shyama tulsi dark tulsi or krishna tulsi dark tulsi.
All the test organisms were screened for their antibacterial activity against leaf and bark extract of azadirachta indica by agar well diffusion method. The 500 mg l1 of extract treated water showed 95 to 98% antibacterial activity in 14 to 16 hrs. In vitro antibacterial activity and minimum inhibitory. Antimicrobial activity of azadirachta indica neem against. Herb ocimum sanctum showed no variation in antibacterial activity after 30 minutes, 1 hr. Antibacterial activity of black pepper piper nigrum linn. May 17, 2016 in vitro antibacterial activity of some plant essential oils. Antimicrobial activity of tulsi oil zone of inhibition mm organisms uw 1w 3w 5w bacillus subtilis 8 staphylococcus aureus 23 14 8 pseudomonas aeruginosa 18 11 3 escherichia coli 10 7 candida albicans 25 17. Individual suspensions of microorganisms were added. Alstonia scholaris is a plant of interest as it is mythologically important in hinduism. The agar diffusion method well or paper disk and the dilution method liquid broth or agar are the two basic techniques used for the examination of the antimicrobial activity of the essential oils.
The antibacterial assay was carried out by microdilution method in order to determine the antibacterial activity of compounds tested against the pathogenic bacteria. Another variety of plant is known as ramatulsi bright tulsi. The antibacterial sensitivity and minimum inhibitory concentration mic of silver nanoparticles was determined against the bacteria. Antibacterial activity of leaf extracts of aloe vera. Antibacterial activity of azadirachta indica neem leaf extract against bacterial pathogens in sudan hala a.
Aqueous, ethanol and acetone leaf and root extracts of ocimum sanctum linn were investigated for their antibacterial activities at various concentrations against escherichia coli, staphylococcus aureus and pseudomonas aeruginosa using standard methods. Antibacterial activity antibacterial activity of the aqueous, alcoholic, chloroform extract and oil obtained from leaves of ocimum sanctum were studied against li, p. Antibacterial effect of herbs and spices extract on. Antibacterial activity of leaf extracts of aloe vera, ocimum sanctum and sesbania grandiflora against the gram positive bacteria jothi karumari r, vijayalakshmi k, tamilarasi l and ezhilarasi balasubramanian post graduate and research department of zoology, ethiraj college for women, chennai8, tamil nadu, india abstract plants, as they. Antimicrobial activity of neem, tulsi, henna and amla against. The aim of this study is to test the antibacterial effect of various herbs and spices on escherichia coli. Faculty of medical laboratory sciences, al neelain university, khartoum, sudan. Pdf comparative study on antimicrobial activity of tulsi.
Bacteria, antimicrobial activity, plant extracts, solvents. Antimicrobial activity of tulsi ocimum sanctum linn. The antiviral activity of tea also was reported john and mukundan, 1979. Phytochemical analysis, antibacterial and antioxidant. Phytochemical analysis, antibacterial and antioxidant activity. Different substances have different extent of antimicrobial activity. The present study deals with the antimicrobial activity of different samples like neem, clove, curry leaves, cardamom, tulsi stem and tulsi leaves. The antibacterial activity of extracts of nine plant species.
Antimicrobial activity of tulsi ocimum tenuiflorum essential oil and. Pankaj goyal1 and purshotam kaushik1 1department of botany and microbiology, gurukul kangri university, hardwar, uttarakhand, india. Antibacterial activity of garlic, tulsi, bitter guard and. Received th may 2011 accepted 28th june 2011 online ready 16th july 2011 abstract. Pdf antibacterial activity of garlic, tulsi, bitter. Antibacterial activity of aqueous, ethanol and acetone. Analysis of variance performed amongst different herbs showed statistically significant difference p tulsi, mint etc. The antibacterial activity of peppermint oil and different extracts of mentha piperita against some grampositive and gramnegative bacterial strains was evaluated in the present research work by agar well diffusion method. Antimicrobial studies indicated that both the acetone leaf and root extracts of o. Studies of antimicrobial properties of different leaf extract.
In present study the antibacterial activity of black pepper and its mode of action on bacteria by membrane leakage studies i. Studies of antimicrobial properties of different leaf. Menthol a non polar terpene can be extracted either by pet. Tulsi is pungent and bitter in taste, pungent in the post digestive effect and has hot potency. The antibacterial activity of ocimum was evaluated by liquid inhibition test.
Tulsi, ram tulasi, dalchini, and timur for potential antibacterial activity against 10 medically important bacterial strains e. Antibacterial activity of the extracts was evaluated by using paper disc agar diffusion method. Tulsi is a sanskrit word which means matchless one. The bacterial suspensions were adjusted with sterile saline to a concentration of 1. Basil or tulsi is significant in the traditional ayurvedic and unani.
Antimicrobial coating of cotton twill tape with neem oil. Materials and methods plant material black pepper piper nigrum berries were purchased from local market. This sacred plant was found in almost every indian household. Tulsi extract against streptococcus mutans and lactobacillus acidophilus an invitro study. Out of 24 plant products evaluated their antibacterial activity against six potato pathogenic bacteria, 18 products exhibited antibacterial activity. Another variety of plant is known as rama tulsi bright tulsi. Antimicrobial activity in leaf extract of neem azadirachta indica against human pathogenic bacteria. The former possesses greater medicinal value and is commonly used for worship. Antibacterial activity of leaf extracts of aloe vera ocimum. Pdf the aim of this study was to evaluate the antimicrobial effects of ethanol.
Pdf antimicrobial activity of tulsi ocimum tenuiflorum essential. Antimicrobial activity of neem, tulsi, henna and amla. Synthesis of silver nanoparticles from the aqueous extract. From both the methods employed for analyzing the antibacterial activity of agnps, it can be inferred that agnps obtained using tulsi extract and quercetin has almost same antibacterial potential. The development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at subclinical doses in poultry and livestock production. The antibacterial activity has been reported to be the upshot of essential oil components, mostly eugenols found in it. Pseudomonas showed resistance to all the solvents except to tulsi leaf ethanol extracts. Antibacterial activity of ocimum sanctum extracts against four foodborne. Rama is also one of the principal avatars of vishnu. Antimicrobial activity of tulsi is due to its constituents, ursolic acid and carvacrol. The proximate, minerals and preliminary phytochemical analysis of ocimum sanctum leaves were studied. Antimicrobial activity of ocimum sanctum leaves extracts and oil.
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